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. Author manuscript; available in PMC: 2016 Aug 4.
Published in final edited form as: Cell Metab. 2015 Jul 23;22(2):291–303. doi: 10.1016/j.cmet.2015.06.021

Figure 6. 2HG Metabolism Is Coupled to Cellular Redox State.

Figure 6

(A and B) NADH/NAD+ ratio determined by enzymatic cycling assay in LF treated with siRNA targeting D2HGDH or L2HGDH expression (A) or overexpressing D2HGDH or L2HGDH (B). (C) F420/F485 fluorescence ratio, corresponding to cytoplasmic NADH/NAD+, in LF treated with permeable analogues of 2OG, D2HG, and L2HG (500 μM) at time 0 normalized to the fluorescence ratio of untreated cells. Data are mean ± SEM. See also Figure S6.

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