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. Author manuscript; available in PMC: 2016 Aug 4.
Published in final edited form as: Cell Metab. 2015 Jul 23;22(2):291–303. doi: 10.1016/j.cmet.2015.06.021

Figure 7. D2HGDH and L2HGDH Silencing Affects Oxidative Phosphorylation and Glycolysis.

Figure 7

(A) Oxygen consumption rate (OCR) of LF treated with siRNA targeting D2HGDH or L2HGDH after treatment with the indicated compounds (Oligo, oligomycin; AMA, antimycin A). (B) Summarized data from (A). (C) Representative micrographs of TMRM-stained LF. Images were inverted and leveled for presentation. Scale bar is 20 μm. Scale bar is 20 μm. (D) Quantification of TMRM staining in normoxia and hypoxia in siRNA-treated LF. (E) Extracellular acidification rate (ECAR) of LF treated with siRNA targeting D2HGDH or L2HGDH before and after treatment with oligomycin to measure glycolytic capacity (2DG, 2-deoxyglucose). (F) Summarized data from (E). (G) Cell-permeable analogues of D2HG and L2HG inhibit oligomycin-stimulated ECAR. Data are mean ± SEM. See also Figure S7.

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