Fig.1. AhR deficiency alleviates HFD-induced adiposity.
Male WT, AhR−/− and AhR+/− mice were fed a NCD or HFD for 14 weeks. (A) Body weight gain during 14-week feeding and appearance. (NCD, WT, n=20, AhR−/−, n=8, AhR+/−, n=6; HFD, WT, n=13, AhR−/−, n=12, AhR+/− HFD, n=14). (B) Epididymal fat pad weight and its percentage of total body weight in NCD-fed or HFD-fed mice at week 14 (NCD, n=4-6; HFD, WT, n=11, AhR−/−, n=7, AhR+/−, n=12). (C and D) Representative images of H&E-stained epididymal white adipose tissue (eWAT) sections from mice with 14 week HFD (C) and adipocyte size eWAT from mice after 14 weeks NCD or HFD (D). (E) Liver was extracted from mice after 14-weeks NCD or HFD, real-time PCR was performed to assess AhR transcript levels. (NCD, n=4-6; HFD, WT, n=13, AhR−/−, n=8, AhR+/−, n=6). *P<0.05, **P<0.001 by two-way ANOVA followed by one-way ANOVA (A), or one-way ANOVA with Turkey's post hoc comparison (B and D).