Figure 1. Generation of Braf^FA mice.

A. Schematic representation of the targeting construct used to generate the Braf^FA mouse through homologous recombination into the endogenous Braf genomic locus of 129SvEv derived ES cells.

B. Schematic of the structure of the Braf^FA allele that expresses normal BRAF and NEO prior to Flp-mediated recombination. P1 and P2 indicate the location of Southern blotting probes used to confirm appropriate targeting of the Braf gene.

C. Schematic of the structure of the Braf^FA allele following Flp-mediated recombination that now expresses mutationally activated BRAF^V600E.

D. Southern blot analysis to identify ES clones with the Braf^FA targeting construct.

E. H&E stained sections of Braf^FA (left) and Braf^CA (right) 10 weeks p.i. with Ad-Flp or Ad-Cre respectively with average tumor burden indicated in the bar graph.

F. Lung sections of tumor-bearing Braf^FA or Braf^CA mice stained with H&E, Ki67, anti-SPC/CC10 or AQP5 using either IHC and IF methods as indicated.

G. Immunoblot analysis of lung tumor lysates from Braf^FA or Braf^CA mice 10 weeks p.i.