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. Author manuscript; available in PMC: 2016 Aug 1.
Published in final edited form as: Cancer Res. 2015 May 22;75(15):3167–3180. doi: 10.1158/0008-5472.CAN-14-3701

Figure 7. Serum growth factors influence the response of cells to inhibition of MDM2.

Figure 7

A. BIA lung cancer cells were treated with vehicle, nutlin-3a, MEK1/2 inhibitor (PD) or the combination in either 0.5%(v/v) or 10%(v/v) fetal calf serum with viable cells stained with Crystal Violet. Photomicrographs of cells in each treatment condition are also provided.

B. Immunoblot analysis of BIA cells grown in either high (10%) or low (0.5%) fetal calf serum and then treated with either control, nutlin, PD or the combination for 6, 15 or 30 hours. (NB: No sample could be harvested from BIA cells treated with the combination of nutlin-3a plus PD for 30 hours in low serum).