Figure 5.

Effects of bilateral microinjection of Ca_V1 channel blockers into the IC on the susceptibility to acoustically evoked seizures following ethanol withdrawal. A. The time course of the effects of bilateral microinjections of ω-agatoxin TK (30 nM/side) showed no significant reduction in the median score of seizure severity from 3 to 2.5, 1 and 1at 0.5, 1 and 2 h post infusion, respectively, compared to pre-infusion (n=6, P<0.2, Kruskal Wallis test). B. Microinjection of ω-agatoxin TK significantly reduced the incidence of WRS and clonus by 33% (P<0.001, χ²) and 67% (P<0.001, χ²), respectively, by 2 h post-infusion during the ethanol withdrawal period compared with pre-infusion. C. The time course of the effects of bilateral microinjections of nifedipine (10 μM/side) showed a significant reduction of the median score of seizure severity from 3 to 2, 0.5 and 0.5 at 0.5, 1 and 2 h post-infusion compared to pre-infusion (n=6, P<0.05, Kruskal-Wallis test). D. Bilateral microinjections of nifedipine significantly reduced the occurrence of WRS and clonus by 50% (P<0.001, χ²) and 83% (P<0.001,, χ²), respectively, by 2 h post-infusion during the ethanol withdrawal. E. The time course of the effects of bilateral microinjections of nimodipine (1 μM/side) showed a suppression of the median score of seizure severity from 3 to 0.5, 0.5 and 0 at 0.5, 1, and 2 h post-infusion during ethanol withdrawal (n=6, P<0.05, Kruskal Wallis test). F. Microinjection of nimodipine significantly reduced the occurrence of WRS (n=6, P<0.001, χ²) and completely suppressed the occurrence of clonus (n=6, P<0.001, χ²) at 2 h post-infusion during the ethanol withdrawal period. Each point and bar graph represents the mean± S.E.M and percentage (%), respectively. *P<0.05, ****P<0.001.