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. Author manuscript; available in PMC: 2016 Sep 1.
Published in final edited form as: DNA Repair (Amst). 2015 Jun 16;33:43–59. doi: 10.1016/j.dnarep.2015.06.003

Figure 6. Schematic of single-turnover protein trap kinetic assay.

Figure 6

This assay is used to determine a rate of unwinding for a given helicase under conditions in which a helicase molecule (monomer or higher order oligomer) that either dissociates from the DNA substrate during the unwinding reaction or completely unwinds the substrate and subsequently dissociates from the unwound strands is prevented from binding and initiating unwinding of another DNA substrate molecule during the incubation period by the presence of excess single-stranded oligonucleotide that serves as a helicase protein trap. See text for details.