Table 3. Stable foci positions.
| Strain | Fluorescent protein | Proportion of cells with foci (%) | N a | ||
|---|---|---|---|---|---|
| 0.15–0.33 Lateral | 0.4–0.5 Central | 0.33–0.40 Neither | |||
| Expected from random positioning on the nucleoid (0.15–0.5) b | (18/35) x 100 51.4 | (10/35) x 100 28.6 | (7/35) x 100 20 | ||
| JJC6632 | dnaX-venus Expo | 60.3 ± 6.6 | 36.6 ± 5.4 | 1.7 ± 3.9 | 476 |
| JJC6632 | dnaX-venus Stat | 68.3 ± 8.1 | 30.4 ± 9.4 | 1.4 ± 1.2 | 221 |
| JJC5956 | dnaX-YPet Expo | 38.3 ± 12.4 | 51.4 ± 10 | 10 ± 7.4 | 574 |
| JJC5956 | dnaX-YPet Stat | 34.8 ± 15.1 | 53.5 ± 11.1 | 11.8 ± 6 | 262 |
| JJC5349 | holD-YPet Expo | 38.7 ± 12.5 | 48.5 ± 8 | 12.8 ± 7.9 | 522 |
| JJC5349 | holD-YPet Stat | 48.9 ± 15.1 | 42.4 ± 5.5 | 8.6 ± 8.2 | 233 |
| JJC6255 | venus-recG Expo | 44.5 ± 9.8 | 41.7 ± 3.1 | 13.7 ± 14.5 | 429 |
| JJC6255 | venus-recG Stat | 44.4 ± 9.7 | 46.3 ± 5.7 | 9.3 ± 6 | 421 |
| JJC6452 | PrrecG-venus Expo | 50.7 ± 11.2 | 34.5 ± 7.8 | 14.8 ± 4 | 258 |
| JJC6220 | venus-rep Expo | 48.9 ± 8.3 | 41.8 ± 5.1 | 9.3 ± 3.2 | 235 |
| JJC6220 | venus-rep Stat | 44.4 ± 3.1 | 44.9 ± 2.7 | 10.8 ± 4.2 | 214 |
a: N is the number of unique stable foci used for ratio determinations.
b: Foci positions were determined by dividing cells in four regions:
-central between 0.4 and 0.5 (region length 0.1)
-lateral between 0.15 and 0.33 (region length 0.18)
-neither central nor lateral between 0.33 and 0.4 (region length 0.07)
-polar between 0 and 0.15 (region length 0.15)
Given that polar foci were extremely rare with all strains, the ratio expected from random positioning in the three remaining regions was calculated by dividing the central, lateral or neither region lengths by the sum of these three region lengths, 0.35.
“Expo” stands for cells in exponential growth. “Stat” stands for stationary phase cells.