Maximum likelihood tree inferred from the partial aa sequence of the gB of the 15 TeHVs strains investigated in this study and of reference strains of other ChHV (ChHV5, -6). Gallid herpesvirus 1 (GaHV1) was included as outgroup. Bootstrap values from 500 iterations are shown. This analysis reveals the existence of at least two distinct genogroups within the TeHV3 genotype (A-green boxed and B-red boxed). A third putative genogroup (C-blue boxed) composed of a single strain is also observed. A TeHV1 strain (IT13/08) clusters within the A genogroup of the TeHV3 genotype. Clear separation of the TeHV3 genogroups from the sequences of other two chelonian herpesviruses (ChHV5 and ChHV6) is observed. (Genbank accession numbers: CH1970/02-KP979727, CH1690/03-KP979726, CH1611/07-KP979721, CH2360/06-KP979725, CH2053/01-KP979723, CH5132/08-KP979729, CH7204/00-KP979719, CH6883/03-KP979730, CH2313/07-KP979722, CH3429/01-KP979720, CH1458/12-KP979718, US1976/98-KP979717, IT186/12-KP979728, IT191/12-KP979716, IT13/08-KP979724, ChHV5-AAU93326, ChHV6-AAM95776, GaHV1-YP_182356).