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. 2015 Aug 5;10(8):e0135080. doi: 10.1371/journal.pone.0135080

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© 2015 Nagatomo et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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Fig 5 — Spectra are CO- and deoxyHb A (A, B), CO- and deoxy-rHb(αH87G) (C, D), and CO- and deoxy-rHb(βH92G) (E, F) between 10 and 16 ppm at pH 7.0 and 25°C. The hemoglobin concentrations of Hb A, rHb(αH87G) and rHb(βH92G) were 1 mM, 800 and 500 μM, respectively, on a heme basis in 0.05 M phosphate buffer (pH 7.0). In addition, rHb(αH87G) and rHb(βH92G) contained 10 mM imidazole.