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. 2015 Aug 5;10(8):e0135080. doi: 10.1371/journal.pone.0135080

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© 2015 Nagatomo et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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Fig 6 — Spectra are between 10 and 30 ppm at pH 7.0 and 25°C. The hemoglobin concentrations of Hb A, rHb(αH87G) an rHb(βH92G) were 1 mM, 800 and 500 μM, respectively, on a heme basis in 0.05 M phosphate buffer (pH 7.0). In addition, rHb(αH87G) and rHb(βH92G) contained 10 mM imidazole.