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. 2015 Aug 5;10(8):e0135080. doi: 10.1371/journal.pone.0135080

Table 2. Summary of conformations of the cavity mutant Hbs and oxygen binding properties of the normal subunits.

rHb(αH87G) rHb(βH92G)
Deoxy Liganded Deoxy Liganded
Quaternary structures
α12 contact regions a T T T R
C-terminal regions b T T T T
Tertiary structure c t r t t
Properties of normal subunits d
νFe-His 217 cm-1 222 cm-1
O2 affinity verylow (T) verylow (T) high (R) high (R)

a, 1H NMR and UVRR;

b, UVRR and near-UV CD (287 nm);

c, near UV CD (294 nm);

d, visible RR and OEC.