Table 2. Summary of conformations of the cavity mutant Hbs and oxygen binding properties of the normal subunits.
rHb(αH87G) | rHb(βH92G) | |||
---|---|---|---|---|
Deoxy | Liganded | Deoxy | Liganded | |
Quaternary structures | ||||
α1-β2 contact regions a | T | T | T | R |
C-terminal regions b | T | T | T | T |
Tertiary structure c | t | r | t | t |
Properties of normal subunits d | ||||
νFe-His | 217 cm-1 | 222 cm-1 | ||
O2 affinity | verylow (T) | verylow (T) | high (R) | high (R) |
a, 1H NMR and UVRR;
b, UVRR and near-UV CD (287 nm);
c, near UV CD (294 nm);
d, visible RR and OEC.