Fig 3. Influence of CD5⁺CD19⁺CD1d^hi B10 cells on IgE production.

B10 cells were sorted from the spleen of AD mice (n = 5) and control mice (n = 5) by flow cytometry. The PBMCs from normal mice and B10 cells from control or AD mice were cultured with LPS (10 μg/mL), PMA (50 ng/mL), and iono (500 ng/mL) for 72h,then the IgElevels were determined in the supernatants by ELISA.PBMCs without B10 cell wereused as a negative control. No differences in IgE levels were observed in the AD and negative control groups, and both groups showed significantly higher IgElevel than that observed in the control group. Data are expressed as mean ± SEM. **P < 0.01, as analyzed by one-way ANOVA, followed by Tukey multiple-comparison test.