Fig. 1.

Analysis of adipocyte size in OM and abdominal SQ AT. (a) Representative images of H&E-stained AT taken from individuals with NGT, GDM or type 2 diabetes (T2DM). Scale bar, 200 μm. (b, c). Size distribution of adipocytes from OM (b) or SQ (c) AT. Plots show the means and SEM at each bin size of women with NGT (blue circles), GDM (red squares) and type 2 diabetes (green triangles). Arrow points to peak in region of larger adipocyte size in tissues from GDM and type 2 diabetes. Insets depict the paired signed rank difference between the histograms. (d) Mean adipocyte size from OM (coloured symbols) and SQ (white symbols) AT from women with NGT (blue circles), GDM (red squares) and type 2 diabetes (green triangles). Symbols show the means of each individual and lines represent the means and SEM of all individuals. *p < 0.05 and ***p < 0.001 for indicated comparisons. (e–h) Linear regression analyses: BMI vs mean OM adipocyte size (r ² = 0.038; p = 0.433) (e); gestational weight gain (GWG) vs mean OM adipocyte size (r ² = 0.149; p = 0.651) (f); mean OM adipocyte size vs serum glucose (r ² = 0.505; p = 0.0009) (g) and maximal OM adipocyte size vs serum glucose (r ² = 0.568; p = 0.0003) (h). White circles, NGT; black circles, GDM