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. 2015 Aug 5;10(8):e0130533. doi: 10.1371/journal.pone.0130533

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© 2015 Kiselar et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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Fig 2 — A-C. Expanded MALDI-TOF MS spectra obtained from rhBD-2 (20ng/μl) incubated at 37°C with 10 μM MGO for 24 h (A), 48 h (B), or 72 h (C) shows increasing peak intensity for a mass increase of + 54 Da (dehydrated) only with concomitant reduction of unadducted rhBD-2 peak intensity. D-F. Expanded MALDI-TOF MS spectra obtained from rhBD-2 (20ng/μl) incubated at 37°C with 100 μM MGO for 2 h (D), 24 h (E), or 48 h (F) shows increasing peak intensities with mass increases of + 54 Da (dehydrated), + 72 Da (intact), and combined ionic species of + 108 Da (+ 54 Da+ 54 Da), + 126 Da (+54 Da + 72 Da), and + 144 Da (+ 72 Da + 72 Da) with time of incubation. Increasing incubation time of the peptide with 100 μM MGO favors mass increases by dehydrated (+ 54 Da) ionic species, only with reduction of unadducted rhBD-2 peak intensity.