Table 3. Real-time qRT-PCR cytokine mRNA expression levels following CAV infection in different lymphoid tissues.
Thymus | Bone marrow | Spleen | Bursa of Fabricius | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
mRNA/day | 4 | 7 | 11 | 14 | 4 | 7 | 11 | 14 | 4 | 7 | 11 | 14 | 4 | 7 | 11 | 14 |
IL-1β | 3.74 1 | -5.25 | -6.11 | - | 1.21 | -2.73 | - | -1.78 | -4.81 | 3.24 | -2.43 | - | - | -1.39 | - | - |
IL-6 | 4.89 | -5.15 | -7.32 | -1.54 | -1.19 | -3.74 | -1.29 | 1.88 | -7.97 | 2.90 | -2.69 | -5.73 | 1.68 | -1.13 | - | -1.96 |
CXCLi2 | -1.31 | 1.62 | - | - | -1.41 | 1.19 | - | 2.47 | -1.74 | -1.99 | -2.32 | -1.94 | 1.15 | - | -8.21 | 1.21 |
IFN-α | 3.46 | -2.52 | -4.64 | -1.39 | -2.22 | -1.85 | 1.41 | 2.16 | -9.95 | - | -1.72 | -4.90 | - | 1.36 | -2.25 | - |
IFN-β | 3.28 | -2.94 | -8.29 | -1.84 | -2.02 | -1.55 | 1.61 | 1.91 | -9.57 | - | -1.70 | -4.05 | - | 1.42 | -3.11 | - |
IL-2 | - | 11.41 | 8.66 | 7.24 | 1.78 | 18.53 | 9.70 | -3.08 | -1.84 | - | -2.43 | -2.75 | - | - | - | - |
IL-12α | - | - | - | - | -7.76 | -3.66 | - | - | - | 1.37 | -1.84 | - | - | - | - | - |
IL-12β | - | - | - | 1.69 | -1.26 | 1.30 | 1.23 | -9.57 | 1.16 | -1.48 | 1.31 | -1.48 | -2.63 | -3.40 | - | 1.30 |
IFN-γ | 1.21 | 2.08 | 2.17 | 2.40 | 1.86 | - | 6.20 | -1.25 | 1.45 | - | -1.59 | -1.15 | 1.62 | -1.98 | -1.89 | -1.37 |
IL-4 | 2.99 | -3.57 | -7.14 | -1.87 | 1.44 | -2.64 | -1.29 | 1.42 | -5.31 | 2.95 | -4.35 | - | - | -1.38 | -2.09 | 1.60 |
IL-13 | 1.35 | 1.65 | 5.36 | 3.31 | 6.01 | - | 15.21 | -1.43 | -1.24 | - | - | - | - | - | - | - |
IL-10 | - | 1.65 | 4.93 | 9.75 | 1.38 | 1.15 | 6.30 | -1.61 | 1.30 | - | - | 1.93 | - | - | - | - |
TGF-β4 | -1.14 | - | 1.92 | 1.30 | - | 1.21 | 1.83 | 1.15 | - | - | -1.33 | -1.18 | -1.69 | -1.22 | 2.20 | 2.55 |
1Mean fold difference between infected and mock-infected birds at each time-point (n = 5 (6 for d 14)); P<0.05, positive values are up-regulated in infected birds and negative values are down-regulated in infected birds.—: no significant difference between groups or mRNA not expressed in one or both groups. Changes greater than three-fold are highlighted in bold.