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. 2015 Aug 5;10(8):e0134842. doi: 10.1371/journal.pone.0134842

Fig 7. Functional evaluation of PPARγ in tumorigenic lung cancer cells.

Fig 7

Growth response and cell migration of lung cancer were analyzed to treatment with PPARγ ligands. (A) Cell growth response. HBEC-C1-wt-PPARγ and HBEC-C1-SUMO-PPARγ cell lines were treated with 3 μM of pioglitazone or troglitazone for 3 days with or without tetracycline induction and followed by cell counting assay. Note that the result represents three independent sets of experiments. Liquid colony formation (B,C) and cell migration (D) assays were performed as described in Materials and Methods. Cells were treated with 3 μM of TZDs, or 3 and 10 μM of WY-14643 under the condition of PPARγ-ON or -OFF. Data represent the mean ± SD (n = 3). Asterisks show statistically significant points as evaluated by ANOVA. *P < 0.001 compared to HBEC-KT control.