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. 2015 Aug 5;10(8):e0134852. doi: 10.1371/journal.pone.0134852

Fig 2. Vancomycin enhances catheter-associated biofilm formation in diabetic mice infected with vancomycin-non-susceptible S. aureus.

Fig 2

(A) Experimental control of catheter-associated biofilm formation assay in mice. Determination of the number of viable biofilm-bound bacteria in subcutaneous catheters removed from healthy/diabetic mice upon infection with VSSA strain ATCC 12598 in the presence of vancomycin treatment. (B) Subcutaneous catheters removed from healthy/diabetic mice upon infection with VRSA strain SJC1200 in the absence/presence of vancomycin treatment. Two representative catheters in each group are shown in the figure. (C) Catheter-associated biofilm materials were observed under SEM. Photos were taken at the indicated magnifications. (D) Determination of the number of viable biofilm-bound bacteria in the removed catheters, as above. (E) Determination of the number of viable biofilm-bound bacteria in the catheters removed from diabetic mice infected with VISA strain Mu50 following vancomycin treatment. (F) The correlation between the blood glucose concentration and catheter biofilm-forming capacity. The capacity was evaluated by determining the number of viable biofilm-bound bacteria in subcutaneous catheters. The number of mice used in each group is shown.