FIG 3 .

Peripheral NKDCs traffic to the CNS after TMEV infection in a VLA-4-dependent manner. (A) Scheme of adoptive transfer experimental design. Congenic B6 CD45.1 splenocytes were transferred intravenously (i.v.) into CD45.2 WT B6 mice 2 days prior to i.c. TMEV infection. Brains and spleens were harvested 3 days p.i. and analyzed for the presence of CD45.1 donor cells. (B) Representative flow plots of CD11c and NK1.1 expression on CD45.1⁺ cells in the brain and spleen of TMEV- or sham-infected mice. (C) The percentage of transferred donor CD45.1⁺ cells and CD45.1⁺ CD11c⁺ NK1.1⁺ is quantitated. (D) Quantification of total CNS lymphocytes and NKDCs (CD3⁻ CD45⁺ CD11c⁺ NK1.1⁺ DX5⁺) 3 days p.i. in TMEV-infected B6 mice treated with the isotype control (black bars) or anti-VLA-4 (white bars) 6 h prior to infection. Data are representative of 2 independent experiments with 5 mice per group. Error bars show standard deviations. ***, P < 0.001; **, P < 0.01; *, P < 0.05.