FIG 4 .
TMEV-activated NKDCs are poor APCs but efficient killers. FACS-purified NK cells, DCs, and NKDCs from TMEV-infected B6 mice were tested for APCs and killing function cocultured with target cells (T cells or YAC-1 cells). (A and B) Splenic DCs and NKDCs were FACS sorted from B6 mice 24 h post-TMEV infection and cocultured at a 1:1 ratio with CFSE-labeled pan-splenic T cells from VP425-38- or VP2121-130-primed mice ± 2 µM cognate peptide. Seventy-two hours later, CD3+ T cells were analyzed for proliferation via CFSE dilution (A) and activation marker expression via expression of CD44 and CD69 (B). (C) YAC-1 target cells were cocultured with FACS-purified NK cells, DCs, and NKDCs from TMEV-infected B6 mice at the indicated effector-to-target ratios for 4 h and assayed for LDH release as a marker of cytotoxicity. Data are representative of 3 independent experiments.