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. 2015 Jul 15;29(14):1524–1534. doi: 10.1101/gad.261792.115

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© 2015 Zheng et al.; Published by Cold Spring Harbor Laboratory Press

This article is distributed exclusively by Cold Spring Harbor Laboratory Press for the first six months after the full-issue publication date (see http://genesdev.cshlp.org/site/misc/terms.xhtml). After six months, it is available under a Creative Commons License (Attribution-NonCommercial 4.0 International), as described at http://creativecommons.org/licenses/by-nc/4.0/.

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Figure 3. — Mutations of key residues in MDM2 impair the ability of RPL11 to up-regulate p53 through MDM2. (A–C) RPL11 cannot fully rescue MDM2 mutant-mediated degradation of p53. Double-knockout cells were transfected with plasmids encoding p53, an increasing amount of Flag-tagged RPL11, and MDM2 wild type or its mutants. Cells were harvested 24 h after transfection, and expression of the indicated proteins was examined by immunoblotting. (D) There was no difference in localization between wild-type MDM2 and its mutants. H1299 cells transfected with Flag-MDM2 or its mutants were immunostained with anti-Flag antibody. Nuclei were counterstained with DAPI. Images were acquired by fluorescence microscopy.