Fig. 4.

Postprandial microbial concentration profiles in caecum of horses fed the hay diet (black) and the oat diet (red). Estimated Multivariate Curve Resolution (MCR) concentration profiles obtained PCR amplification of the variable V3 and V4 regions of 16S rRNA gene, direct sequencing, and automatic capillary electrophoresis. Main resolved bacterial groups of the mixed sequences at the family level were Porphyromonadaceae, Prevotellaceae, and Lachnospiraceae. Two of the components (Mixed 1 and 2) could not be resolved at a family level, but belong to the phylum Proteobacter. Error bars represent standard deviations.