Table 2.
Primers used in this study.
| Primer | Sequence (5′–3′), restriction sites are in bold | Final construct |
|---|---|---|
| SP/Bc-CIISA/XhoI | TCATCATCACTCGAGGAAATATGGGGTGCGAAATT | pET15b-Bc-HCIISA |
| ASP/Bc-CIISABamE | TCATCATCAGGATCCTTTACTAAAATAACTATGAT | |
| SP/BcIISB/NdeI | TCATCATCACATATGGTGATTGTAGTGGTAAAAGA | pETDuet-Bc-IISC |
| ASP/BcIISB/XhoI | TCATCATCACTCGAGTACACTTATGATTTTCTTTT | |
| SP/IISA/NcoI | TCATCATCACCATGGATGATCTCTAACATTCGAAT | pBADIISAB Bc |
| ASP/IISB/HindIII | TCATCATCAAAGCTTGCAAATGTAGAAAGAGTGTA | |
| SP/IISCBc/PstI | TCATCATCACTGCAGTGAAAAGGGGGAGAAGAGATG | pBADIISC Bc |
| ASP/IISCBc/HindIII | TCATCATCAAAGCTTATGCTCTATGCATTTTCTTT | |
| SP/IISABc/EcoRI | TCATCATCAGAATTCATGATCTCTAACATTCGAAT | pBADIISAC (via pBADIISC Bc) |
| ASP/IISABc/NcoI | TCATCATCACCATGGTAGAAGAAAAGGACAGAAAA | |
| SP/CIISABc/BamHI | TCATCATCAGGATCCCGAAATATGGGGTGCGAAATT | All four BACTH vectors carrying CIISA Bc |
| ASP/CIISABcSTOP/EcoRI | TCATCATCAGAATTCGATTCTGTCCTTATTTACTA | pUTCCIISA Bc, pKTCIISA Bc |
| ASP/CIISABcNOSTOP/EcoRI | TCATCATCAGAATTCGATTTACTAAAATAACTATGA | pUTCIISA Bc, pKNTCIISA Bc |
| SP/IISBBc/BamHI | TCATCATCAGGATCCCGTGATTGTAGTGGTAAAAGA | pUTIISB Bc, pUTCIISB Bc |
| ASP/IISBBcNOSTOP/EcoRI | TCATCATCAGAATTCGATGATTTTCTTTTTAATTCTT | pUTIISB Bc |
| ASP/IISBBcSTOP/EcoRI | TCATCATCAGAATTCGAGCAAATGTAGAAAGAGTGTA | pUTCIISB Bc |
| SP/IISCBc/BamHI | TCATCATCAGGATCCCATGGCTGAAGTCAATGTGCA | All four BACTH vectors carrying SpoIISC Bc |
| ASP/IISCBcNOSTOP/EcoRI | TCATCATCAGAATTCGATGCATTTTCTTTTGTTCTTT | pUTIISC Bc, pKNTIISC Bc |
| ASP/IISCBcSTOP/EcoRI | TCATCATCAGAATTCGACTATGCATTTTCTTTTGTTC | pUTCIISC Bc, pKTIISC Bc |
| SP/CIISABc/BamHI2 | TCATCATCAGGATCCGATTTCAGAAATATGGGG | pETDuetCIISA Bc |
| ASP/CIISABc/EcoRI | TCATCATCAGAATTCGATTCTGTCCTTATTTACTAA | |
| SP/IISBBc/KpnI | TCATCATCAGGTACCGTGATTGTAGTGGTA | pETDuetIISB Bc, pETDuetCIISAB Bc (via pETDuetCIISA Bc) |
| ASP/IISBBc/XhoI | TCATCATCACTCGAGTGATTTTCTTTTTAA | |
| SP/IISABs/EcoRI | TCATCATCAGAATTCATGGTTTTATTCTTTCAGATCATGGTCTGG | pBADIISA Bs, pBADIISAB Bs |
| ASP/IISABs/NcoI | TCATCATCACCATGGTTCCATTATCCTTCACCTTC | pBADIISA Bs |
| ASP/IISBBs/NcoI | TCATCATCACCATGGTTTAGTGTGATCATGCTTTT | pBADIISAB Bs |
| SP/IISCBs/PstI | TCATCATCACTGCAGAGAGGATAATGTCAGGTGAT | pBADIISAC Bs |
| ASP/IISCBs/HindIII | TCATCATCAAAGCTTCAAAGACCATAAAAATCCCGGAGCCGCTCC | |
| SP/CIISABs/BamHI | TCATCATCAGGATCCCAAAAAACTGGCCGGCAGCGAGCTTGAAACA | All four BACTH vectors carrying CIISA Bs |
| ASP/CIISABsSTOP/EcoRI | TCATCATCAGAATTCTTATCCTTCACCTTCCTCCT | pUTCCIISABs, pKTCIISABs |
| ASP/CIISABsNOSTOP/EcoRI | TCATCATCAGAATTCGATCCTTCACCTTCCTCCTCAA | pUTCIISABs, pKNTCIISABs |
| SP/IISBBs/BamHI | TCATCATCAGGATCCCATGGAACGTGCGTTTCAAAACAGATGCGAG | pUTIISB Bs, pUTCIISB Bs |
| ASP/IISBBsNOSTOP/EcoRI | TCATCATCAGAATTCGATCCTTCACCTTCCTCCTCAA | pUTIISB Bs |
| ASP/IISBBsSTOP/EcoRI | TCATCATCAGAATTCTCATGCTTTTTTTCGTTTAT | pUTCIISB Bs |
| SP/IISCBs/BamHI | TCATCATCAGGATCCCGTGACATATAATAAATACAA | All four BACTH vectors carrying SpoIISC Bs |
| ASP/IISCBsNOSTOP/EcoRI | TCATCATCAGAATTCGATGCTTTTTTTCGTTTATACT | pUTIISC Bs, pKNTIISC Bs |
| ASP/IISCBsSTOP/EcoRI | TCATCATCAGAATTCGATTATTTTTTCTTCTTCAACT | pUTCIISC Bs, pKTIISC Bs |