Figure 6. Inhibition of miR-29 alleviates miR-29-mediated repression of the anti-lipogenic transcription factor Ahr.

(a) Densitometry analysis for hepatic Ahr protein from LNA29-treated mice (n = 5) compared to saline-treated controls (n = 3). β-actin was used as the loading control. Immunoblot results also shown for two representative mice from each treatment group. (b) Expression fold-change (FC) by RNA-seq in LNA29-treated mice (n = 6) relative to saline-treated controls (n = 6) is shown for genes known to be transcriptionally activated by Ahr. p-values were calculated by one-tailed unpaired Student’s t-test. (c) A diagram of the predicted base pairing between miR-29 and the Ahr 3′ UTR sequence of both mouse and human is shown. (d) Effects of miR-29a mimic (10 nM) in HEK293T cells on the activity of Firefly (FL) luciferase with or without the Ahr 3′ UTR normalized to Renilla luciferase (RL) are shown. ***p < 0.005; *p < 0.05; p-values were calculated by two-tailed unpaired Student’s t-test. Error bars represent standard error of the mean.