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. 2015 Aug 6;5:12889. doi: 10.1038/srep12889

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Copyright © 2015, Macmillan Publishers Limited

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(a) Flow cytometry analysis using Annexin V-FITC/PI double staining. Compound 7 and ISJ induced apoptosis in HepG2 cells. (b-c) Antivascular activities of compound 7 and ISJ. Treatment with compound 7 and ISJ at 10 μM for 24 h resulted in the inhibition of HepG2 migration and invasion. (b) Wound healing assay (magnification × 50). (c) Matrigel invasion assay (magnification × 100). (d) Cell migration was quantified by measuring the distance between the two boundaries of the acellular area, and the result was expressed as a ratio to DMSO-treated cells (control). Data are shown as the mean ± SD of three independent experiments performed in triplicate. *P < 0.05. (e) Cell invasion was quantified by counting the mean number of invaded cells (±SD) under the microscope in three randomly selected fields. *P < 0.05.