Fig. 2.

Embryo-specific expression of CLE19 during seed development. (A–E) GUS expression in seeds excised from pCLE19:GUS:tCLE19 transgenic plants. Note that GUS staining was first detected in late globular-stage embryos (B), and persisted in the triangular (C), heart-shaped (D), and torpedo-stage embryos (E), while no GUS expression was observed in the early globular embryo (A). Scale bars=50 μm. Early embryos (A–C) are indicated by arrowheads; h, heart-shaped embryo; t, torpedo-stage embryo; e, endosperm; s, suspensor. (F) RT–PCR to show CLE19 expression in ovules and embryos, but not in mixed endosperm and testa tissues (En+Ts). EIF4A was used as an internal standard. Control, without cDNA.