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. 2015 Jun 12;66(17):5217–5227. doi: 10.1093/jxb/erv293

Fig. 7.

Fig. 7.

Prolonged and elevated expression of early endosperm-specific genes in the endosperm of pCLE19:CLE19 G6T :tCLE19 transgenic plants. (A) qRT-PCR analyses showed elevated expression of MEA, FIS2, and AGL62 in those abnormal ovules (a) from a pCLE19:CLE19 G6T :tCLE19 transgenic plant (CLE19 G6T), as compared with those normal ovules (n) from the same plant, and ovules from the wild type (WT). All ovules were analysed at 12 DAP. Data represent the mean ±SD of three independently extracted RNA samples. (B–E) GUS expression in seeds from the wild type (B) and pCLE19:CLE19 G6T :tCLE19 transgenic plants carrying a pMEA:GUS reporter construct, examined at 12 DAP (C–E). Note that aborted seeds in (D) and (E) showed prolonged GUS expression in chalarzal endosperm (marked by asterisks), but not in the normal seed (C) in the same plant. Embryos are traced with dotted lines. c, cotyledon; hy, hypocotyl; h, heart-shaped stage embryo; t, torpedo-stage embryo. Asterisks indicate significant differences from the wild-type (P<0.01 by Student’s t-test). Scale bars in B–E=50 μm.