Figure 2. Effects of inducible loss of PTEN in adipocytes during high-fat diet exposure.

(a) Body weights of mice fed a doxycycline (600 mg kg⁻¹) high-fat diet starting at 6 weeks of age for the subsequent 42 days (N=5 for each group). (b) Concentration of glucose in blood of mice gavaged with 2.5 g kg⁻¹ glucose body weight (n=5 for each group, average area under the curve—control=37,827, AiPKO=22877, P=0.0011). (c) Serum insulin concentrations of mice during oral glucose tolerance test shown in (b). (d) Blood glucose levels in mice following injection of the β3-adrenergic agonist CL316,243 at 0.5 mg kg⁻¹ (n=4 control and 5 AiPKO). (e) NEFA (f) free glycerol and (g) insulin levels in the mice from d. (h) Blood glucose levels displayed as a percent of fasting following injection with insulin (0.5 IU kg^−I). (i) Inflammatory gene expression in adipose tissue from mice fed a HFD doxycycline-containing diet for 6 weeks (N=3 for each group). All data compare control mice with AiPKO mice. For all points, *P<0.05, **P<0.01, ***P<0.001 (Student's T-test). All data are mean±s.e.m.