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. Author manuscript; available in PMC: 2016 Feb 5.
Published in final edited form as: Mol Cell. 2015 Jan 15;57(3):479–491. doi: 10.1016/j.molcel.2014.12.018

Figure 6. The Mre11 nuclease function is critical for rad50-46 DSB end resection and damage survival.

Figure 6

A. HO-DSB end resection in G2 arrested cells at 30°C in a time course over 210 minutes in wild-type, rad50-46 and rad50Δ cells. 5′-to-3′ end resection products of an HO-DSB are detectable with an ssRNA probe after alkaline gel electrophoresis of SspI-digested DNA. The migration levels of HO-uncut (1.1 kb), HO-cut (0.9 kb), and resection fragments r1 (1.7 kb), r2 (3.5 kb), r3 (4.7 kb), r4 (5.9 kb), r5 (6.5 kb), r6 (8.9 kb) and r7 (15.8 kb) from the HO-site (HO) are indicated. Right panel: Quantification of the r2, r3 and r4+r5 fragments of the southern blots shown. Similar results were obtained in three experiments. Note that the blots and quantifications for wild-type and rad50Δ were previously published elsewhere (Hohl et al., 2011). B. Damage survival of rad50-46 is dependent on Mre11 nuclease function and Sae2.