Fig. (1).

Celastrol inhibits neutrophil oxidative burst induced by TNF#x03B1; or immune complexes. A, Representative flow cytometry histograms showing inhibition of TNFα or Ova IC-induced oxidative burst by celastrol (5 µM). The red vertical lines represent the peak fluorescence intensity of Rhodamine 123 in cells treated with vehicle only (as the base level of oxidative burst). B, A summary showing that celastrol (5 µM) significantly inhibited TNFα or Ova IC-induced oxidative burst. The relative levels of oxidative burst were presented as fold changes of mean fluorescence levels of Rhodamine 123 compared to non-stimulated neutrophils (vehicle only). The data represent at least five independent experiments with neutrophils from different donors. *p<0.05 and ***p< 0.001 by ratio paired t-test. C, A diagram showing dose-dependent inhibition of TNF#x03B1; and Ova IC-induced oxidative burst by celastrol. Data were plotted as the mean ± SD and represented at least three independent experiments.