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. Author manuscript; available in PMC: 2015 Aug 6.
Published in final edited form as: Handb Exp Pharmacol. 2014;223:1143–1176. doi: 10.1007/978-3-319-05161-1_18

Figure 3.

Figure 3

Inhibition of TRPM8 by reduction of PI(4,5)P2 in intact cells, right panels in A,B and C show cartoon of the method used. A. Whole cell patch clamp measurement from (Daniels et al., 2009) showing inhibition of menthol induced RPM8 currents by the pharmacological PLC activator m-3M3FBS. B. Inhibition of TRPM8 activity by the rapamycin inducible 5-phosphatase from (Varnai et al., 2006). C. Inhibition of TRPM8 activity by depolarization-induced depletion of PI(4,5)P2 in ciVSP expressing cells from (Yudin et al., 2011). Left panel shows a measurement with the active ciVSP, middle panel with an inactive mutant.