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. 2015 Aug 7;22(1):65. doi: 10.1186/s12929-015-0171-x

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© Huang et al. 2015

Open Access This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 1 — The expression and purification of rlipoA-RBD. The expression and the purity of rlipoA-RBD were confirmed by SDS-PAGE (a) and western blot with TcdA-specific monoclonal antibody (b). The purification of rlipoA-RBD as shown in panel a, lanes 2 to 4 were crude extract loaded on the first Ni-affinity column, eluent of 200 and 500 mM imidazole, respectively. The purity of rlipoA-RBD obtained from IMAC were confirmed by SDS-PAGE (panel a, lane 5) and western blot with TcdA-specific monoclonal antibody (panel b, lane 7). The first lane in each panel was the molecular markers