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. 2015 Aug 7;22(1):65. doi: 10.1186/s12929-015-0171-x

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© Huang et al. 2015

Open Access This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 3 — Up-regulation of surface biomarkers of BMDC by rlipoA-RBD. BMDC from C57BL/6 was collected and treated with GM-CSF on days 0 and 3. A-rRBD and rlipoA-RBD were treated on day 6 for 18 h, then DC were collected to analyze their surface markers, including CD-40 (a), CD-86 (b), CD-80 (c), and MHC-II (d) by flow cytometry. All groups were divided into polymyxin B (PMB) treated (black-net bar) or without (white bar) to validate insignificant LPS contamination. All surface marker signaling was normalized by calculating the ratio of mean fluorescence intensity (MFI) between medium control and treatments. The experiments had been performed at least three times