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. 2015 Aug 7;22(1):65. doi: 10.1186/s12929-015-0171-x

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© Huang et al. 2015

Open Access This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 9 — Adjuvant effect in hamster challenge model studies. Panel a, five groups of hamsters (n = 6) were gastrically inoculated with 100 CFU. of C. difficile (the dose can kill >50 % of hamsters) at 2 weeks after the third immunization with either PBS, A-rRBD + B-rRBD, rlipoA-RBD + B-rRBD, or A-rRBD + B-rRBD formulated with alum (300 μg) or Pam3CSK4 (10 μg). The final survival rates were reported. Panel b, The number of C. difficile colonies (CFU) grown in TCCFA selective medium. Bacterial colonies obtained from the faeces (~100 mg) of survived hamsters were determined every two days after challenge. The number of CFU shown in the figure represented the geometric mean of CFU per 100 mg of feacal pellets obtained from survived hamsters in each group after the spore challenge