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. 2015 Aug 1;8(8):805–815. doi: 10.1242/dmm.019935

Fig. 1.

Fig. 1.

Gpa33−/− mice do not exhibit steady-state gastrointestinal pathology. (A) Representative histological images of small intestine and colon from 10-week-old Gpa33−/− and WT mice on a pure C57Bl/6 background. H&E stains all epithelial cells, Periodic acid-Schiff stains the mucins in goblet cells pink (arrows), and phloxine and tartrazine highlights the granules in Paneth cells (arrows). (B) No difference in proliferation is observed in colonic crypts of Gpa33−/− and WT mice. Representative BrdU immunohistochemistry in the colon of 10-week-old Gpa33−/− and WT mice collected 2 h after intraperitoneal BrdU injection (100 mg/kg). (C) Quantitation of BrdU-positive cells expressed as a fraction of total cells per crypt. A minimum of ten crypts were evaluated per mouse. (D) Both Gpa33−/− and WT mice exhibit mild progressive colitis with age. Representative images of H&E-stained distal colon sections from mice aged 3, 6 and 12 months. Arrows indicate sub-mucosal thickening and immune cell infiltration, characteristic of colitis. (E) Quantitation of colitis using the scheme shown in supplementary material Table S1. Mean±s.e.m., n=3. Scale bars: 50 µm.