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. 2015 Aug 1;8(8):891–902. doi: 10.1242/dmm.020214

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© 2015. Published by The Company of Biologists Ltd

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0), which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed.

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Fig. 3. — Motor domain mutations disrupt dynamics of endocytic actin patches visualized with mCherry-tagged fimbrin (Fim1). (A-F) Time courses of (upper panels) average fluorescence intensity of (green, solid circles) wild-type (WT) and mutant Myo1-mGFP and (red, open squares) Fim1-mCherry in actin patches, and (lower panels) raw (thin lines) and average (thick lines) distances traveled by Fim1-mCherry or Myo1-mGFP in patches. (G-I) Bar graphs showing (G) the total lifetime, assembly time and disassembly time, (H) percentage of internalization, and (I) mean peak intensities of Fim1-mCherry patches in wild-type and mutant myo1 strains. Note increased total lifetime, assembly and disassembly times, and decreased internalization of Fim1-mCherry patches in the T140I, A181P and G308R myo1 mutant, and Δmyo1 strains. In all panels, error bars represent s.d. (A-G,I) n=5-12 patches. (H) n=23-43 patches in 3-6 cells. The asterisks indicate statistical significance: *P<0.05; **P<0.01.