Fig. 4.

Citrobacter colitis results in greater mortality and lower levels of serum pro-inflammatory cytokines in OPTN-deficient mice. (A,B) Optn^+/+ (grey) and Optn^−/− (black) mice were gavaged with live Citrobacter rodentium and followed for 9 days to assess (A) weight loss and (B) mortality (n=29-31 mice/genotype over 5 experiments). (C) Serum TNF, (D) IL6 and (E) CXCL1 were measured in naïve mice, day 2 and day 9 after Citrobacter inoculation, in tail bleed and cardiac puncture serum (n=5 mice/group). Results shown are mean±s.e.m. (*P<0.05, **P<0.01 and ***P<0.001; two-tailed, unpaired t-test and logrank test).