Figure 2. Histology and genomic view of prostates from Pten null (Pb-Cre⁺;Pten^L/L) mice.

A-A’, representative microscopic images from hematoxylin and eosin-stained sections of prostate glands from which DNA was extracted for CGH hybridization shown at low (4×) and high-power (20×) magnification, respectively. B, Genomic DNA from Pb-Cre⁺;Pten^L/L prostate tumors and germline spleen were hybridized against sex-matched normal mouse C57BL/6 reference DNA using Agilent CGH slides containing 180K probes. Whole genome view of overlaid moving averages (2 Mb window) for the log2 ratios of fluorescence between a sample/reference DNA probe (Y axis) plotted at its genomic position (X axis), red, blue and yellow (tumors; n = 3) and green shades (germline n = 3). Aberrations called by the ADM-2 algorithm are identified by horizontal bars. (^*) Aberrations called by the ADM-2 algorithm in a single sample, that by manual inspection exhibited similar fluorescence intensities in all samples including germline and tumor (see Supplemental Figure S4, for representative examples). (¹) CNAs in common between germline, and Pten lesions that are not visually obvious in the genome view window.