Table 1.
Primers and probes for RT-PCR.
| Gene | Product size | Primer/probe | Sequence |
|---|---|---|---|
| RAGE | 133 bp | Forward | 5′ AAAGCTTTCGGGAGTGGTCAGACT 3′ |
| Reverse | 5′ ATCTGTCTTCTTGCTC GCAGGGAT 3′ | ||
| IR | 182 bp | Forward | 5′ TTTGGGAAATCACCAGCTTGGCAG 3′ |
| Reverse | 5′ AATCTCCAGG AAGGTTGGCCTCAT 3′ | ||
| IGF-1 | 423 bp | Forward | 5′TGAAGATGCACA CCATGTCCTCCT3′ |
| Reverse | 5′TGCACTC CCTCTA CTTGCG TTCTT 3′ | ||
| IGF-1R | 267 bp | Forward | 5′ GCGGCTGGAAACTCTTCTACAACT 3′ |
| Reverse | 5′ TGGTCTT CTCACACATCGGCTTCT 3′ |
Abbreviations: RT-PCR: reverse transcription polymerase chain reaction; RAGE: receptor for advanced glycation end-products; IR: insulin-receptor; IGF-1: insulin-like growth factor-1; IGF-1R: insulin-like growth factor-1 receptor.