Enzymes – Horseradish Peroxidase (HRP) |
Horseradish peroxidase (HRP) |
Bulk loaded silk films |
+ |
+ |
- |
Qian et al., 1996 |
Bulk loaded silk films |
+ |
+ |
- |
Qian et al., 1997 |
Bulk loaded silk films |
+ |
- |
+ |
Hofmann et al., 2006 |
Chemical coupling or adsorption onto silk sponges |
+ |
+ |
+ |
Vepari and Kaplan, 2006 |
Microparticle encapsulation |
+ |
- |
+ |
Wang et al., 2007 |
Bulk loaded silk films (nanopatterned) |
+ |
- |
- |
Lawrence et al., 2008 |
Bulk loaded silk films |
+ |
+ |
- |
Lu et al., 2009 |
Bulk loaded silk films |
+ |
+ |
+ |
Lu et al., 2010 |
Bulk loaded silk films (microneedle arrays) |
+ |
- |
+ |
Tsioris et al., 2011 |
Enzymes – Glucose Oxidase (GOx) |
Glucose oxidase (GOx) |
Bulk loaded silk films |
+ |
+ |
- |
Kuzuhara et al., 1987 |
Bulk loaded silk films |
+ |
+ |
+ |
Demura and Asakura, 1989 |
Bulk loaded silk films |
+ |
+ |
+ |
Demura et al., 1989 |
Bulk loaded porous silk films |
+ |
- |
- |
Demura and Asakura, 1991 |
Bulk loaded silk films |
+ |
+ |
+ |
Zhang et al., 1998 |
Bulk loaded silk films and blended silk-PVA films |
+ |
+ |
+ |
Liu et al., 1996 |
Bulk loaded silk films |
+ |
+ |
- |
Lu et al., 2009 |
Other Enzymes |
Lipase |
Glutaraldehyde crosslinking onto silk fibers |
+ |
+ |
+ |
Chatterjee et al., 2009 |
Bulk loaded silk films |
+ |
+ |
- |
Lu et al., 2009 |
Adsorption onto silk woven fabric |
+ |
+ |
+ |
Chen et al., 2010 |
Cholesterol oxidase |
Chemical coupling onto woven silk substrates |
+ |
+ |
- |
Saxena et al., 2010 |
Tyrosinase |
Bulk loaded silk films |
+ |
+ |
+ |
Acharya et al., 2008 |
Ribonuclease |
Chemical coupling onto woven silk substrated |
+ |
+ |
- |
Cordier et al., 1982 |
Alkaline phosphatase |
Chemical coupling or adsorption onto woven silk substrates |
+ |
- |
- |
Grasset et al., 1983 |
β-glucosidase |
Bulk loaded silk films |
+ |
+ |
+ |
Miyairi et al., 1978 |
Uricase |
Bulk loaded silk films |
+ |
+ |
+ |
Zhang et al., 1998 |
Heme proteins (myoglobin, hemoglobin, horseradish peroxidase, and catalase |
Bulk loaded silk films |
+ |
+ |
+ |
Wu et al., 2006 |
L-asparaginase (ASNase) |
Glutaraldehyde crosslinking to silk powder |
+ |
+ |
+ |
Zhang et al., 2005 |
Phenylalanine ammonia-lyase (PAL) |
Encapsulation silk particles |
+ |
+ |
+ |
Inoue et al., 1986 |
Invertase |
Bulk loaded silk hydrogel, lyophilized, then ground to powder |
+ |
+ |
- |
Yoshimizu and Asakura, 1990 |
Organo-phosphorus Hydrolase (OPH) |
Bulk loaded silk films |
+ |
+ |
+ |
Dennis et al., in preparation |
Growth Factors |
BMP-2 |
Adsorption onto silk films or covalent coupling onto silk films |
+ |
- |
+ |
Karageorgiou et al., 2004 |
Adsorption onto 3D porous silk sponge |
+ |
- |
+ |
Karageorgiou et al., 2006 |
Bulk loaded in electrospun silk nanofiber mats |
+ |
- |
+ |
Li et al., 2006 |
Adsorption onto 3D porous silk sponge |
+ |
- |
+ |
Kirker-Head et al., 2007 |
Encapsulation in silk microspheres |
+ |
- |
+ |
Bessa et al., 2010 |
Bulk loaded silk hydrogels (gelation induced via sonication of aqueous silk solution) |
+ |
- |
+ |
Diab et al., 2011 |
FGF-2 |
Adsorption onto silk films (retention enhanced by chemical decoration of fibroin with sulfonic acid moieties) |
+ |
- |
+ |
Wenk et al., 2010 |
IGF-I |
Bulk loaded porous 3D scaffolds |
+ |
- |
+ |
Uebersax et al., 2008 |
Encapsulation in silk fibroin microspheres |
+ |
- |
+ |
Wenk et al., 2008 |
PTH |
Chemical coupling to silk films |
+ |
- |
+ |
Sofia et al., 2001 |
bFGF |
Adsorption onto 3D porous silk sponges (aqueous- or HFIP-derived) |
- |
- |
+ |
Wongpanit et al., 2010 |
NGF |
Bulk loaded porous tube-shaped scaffolds f |
+ |
- |
+ |
Uebersax et al., 2007 |
GDNF NGF |
Bulk loaded silk film tubes |
+ |
- |
+ |
Madduri et al., 2010 |
BMP-2 IGF-I |
Lipid template silk fibroin microspheres incorporated into aqueous-derived silk porous scaffolds using a gradient process |
+ |
- |
+ |
Wang et al., 2009 |
BMP-2 VEGF |
Bulk loaded silk hydrogels (gelation induced via sonication of aqueous silk solution) |
+ |
- |
+ |
Zhang et al., 2011 |
Other Proteins |
Low mol. wt. Protein A (LPA) |
Bulk loaded silk films |
+ |
+ |
+ |
Kikuchi et al., 1999 |
Hemoglobin |
Bulk loaded silk films |
+ |
- |
- |
Lawrence et al., 2008 |
IgG |
Immobilization and enrichment on the silk film surface |
+ |
+ |
- |
Lu et al., 2011 |
Green Fluorescent Protein (GFP) |
Bulk loaded silk films |
+ |
- |
- |
Putthanarat et al., 2004 |
NeutrAvidin |
Chemically coupled to silk microspheres and silk protein in solution |
+ |
- |
+ |
Wang et al., 2011 |
Insulin |
Coupled to silk fibroin powder gluteraldehyde |
+ |
- |
+ |
Zhang et al., 2006 |
Murine anti-TGFb IgG1 monoclonal antibody |
Bulk loaded sonication-induced silk hydrogels |
+ |
- |
- |
Guziewicz et al., 2011 |
Small Molecules |
Adenosine |
Encapsulated into silk microspheres loaded into silk sponges and coated with drug-loaded silk films |
+ |
- |
+ |
Wilz et al., 2008; Szybala et al., 2009
|
Chlorophyll a β-carotene Astaxanthin |
Adsorption onto silk powder |
+ |
+ |
- |
Ishii et al., 1995 |
Oleuropein Rutin |
Adsorption onto silk powder |
+ |
- |
+ |
Bayçin et al., 2010 |
Doxycycline Ciprofloxacin |
Drugs “dyed” onto silk fibers |
+ |
- |
+ |
Choi et al., |
Curcumin |
Encapsulated with silk nanoparticles |
+ |
- |
+ |
Gupta et al., 2009 |
Penicillin, Tetracycline Rifampicin Erythromycin |
Bulk-loaded silk films and adsorption |
+ |
+ |
+ |
Pritchard et al., in preparation |
Tetracycline |
Bulk loaded silk films |
+ |
- |
+ |
Tsioris et al., 2011 |
Doxorubicin |
Bulk loaded silk films |
+ |
+ |
- |
Tao et al., submitted |
Phenolsulfon-phthlein (Phenol Red) |
Bulk loaded silk films |
+ |
- |
- |
Lawrence et al., 2008 |
4-amino-benzoic acid |
Chemically coupled to silk films |
+ |
- |
- |
Tsioris et al., 2010 |