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. Author manuscript; available in PMC: 2016 Aug 4.
Published in final edited form as: Biochemistry. 2015 Jul 21;54(30):4652–4664. doi: 10.1021/acs.biochem.5b00573

Figure 1.

Figure 1

The optical changes during a single turnover with benzoate are well-accounted for by linear summations of the spectra of the reduced and oxidized Rieske cluster. Selected optical spectra observed after reduced BZDO (100 µM) was mixed with an O2 saturated reaction buffer containing benzoate (20 mM) at 4 °C in a stopped-flow spectrophotometer. The spectra are combinations of 21 single wavelength time courses collected from 300 −725 nm. The simulated spectra were calculated by summing the indicated fractions of the fully reduced and fully oxidized Rieske cluster spectra.