. Author manuscript; available in PMC: 2016 Aug 4.

Published in final edited form as: Biochemistry. 2015 Jul 21;54(30):4652–4664. doi: 10.1021/acs.biochem.5b00573

Table 2.

Kinetic parameters from substrate and O₂ concentration dependence of product coupled phase RRT-1^a

	K_d,substrate (µM)	k_forward (s⁻¹)	K_d,O2 (µM)	k_forward (s⁻¹)
Benzoate	191 ± 10	213 ± 4	135 ± 13	212 ± 6
4-FB	226 ± 40	5.6 ± 0.2	163 ± 12	6.4 ± 0.1
3,5-FB	950 ± 140	17 ± 1	150 ± 14	15 ± 0.4
3,4,5-FB	1800 ± 200	1.1 ± 0.1	152 ± 7	0.89 ± 0.02

Apparent K_d values determined from hyperbolic fits to the data shown in Figures 4 and S2. The value for k_reverse is approximately zero in all cases.