Table I.
Delivery of phIL-15 by electroporation results in long-term survival and resistance to challenge. Mice that remained tumor free after the primary inoculation of B16.F10 melanoma cells and delivery of pDNA using electroporation were challenged on the opposite flank with a subcutaneous injection of B16.F10 melanoma cells. They were monitored for an additional 50 days. Data represents animals that were tumor free 50 days post challenge.
| Challenge | ||||
|---|---|---|---|---|
| Total animals (n) | Day 0 (n) | Day 50 (n) | Overall survival (%) | |
| phIL-15 injection | 12 | 0 | 0 | - |
| pVAX1 + 1300 V/cm | 14 | 6 | 3 | 21 |
| phIL-15+1300 V/cm | 11 | 11 | 5 | 45 |
| pVAX1+500 V/cm | 13 | 9+ | 3 | 23 |
| phIL-15+500 V/cm | 12 | 7* | 5 | 42 |
+Of the 10 surviving animals in this group one was found dead on day 48.
*There was one mouse in this group that had a small tumor on day 51 that completely regressed by day 0 of challenge and was included in the challenge.