Figure 2.

(A) Temporal expression patterns of eight neuroinflammatory genes in the MPI^118Q/118Q cerebellum. These eight genes (Clec7a, Cd68, Tyrobp, Trem2, Gfap, Ly86, Pycard and Fcgr3) were selected from the microarray data of the MPI^118Q/118Q KI mice. The qPCR data were evaluated using the ΔCt method. The statistically significant differences were detected for Clec7a (5, 6 and 8 weeks, P < 0.02, <0.05 and <0.02, respectively), Cd68 (5, 6 and 8 weeks, P < 0.05, <0.05 and <0.02, respectively), Gfap (5, 6 and 8 weeks, P < 0.05, <0.05 and <0.02, respectively), Fcgr3 (5, 6 and 8 weeks, P < 0.05, <0.05 and <0.01, respectively), Ly86 (6 and 8 weeks, P < 0.05 and <0.01, respectively), Tyrobp (8 weeks, P < 0.05), Trem2 (8 weeks, P < 0.01) and Pycard (8 weeks, P < 0.02) by Student's t-test. (B) The expression of eight neuroinflammatory genes (Clec7a, Cd68, Tyrobp, Trem2, Gfap, Ly86, Pycard and Fcgr3) in the Sca6^84Q/84Q KI cerebellum at 90 weeks of age. The error bars represent standard errors. The asterisks indicate a statistically significant difference (P < 0.05). (C) Immunohistochemical detection of Iba1-positive microglia in the 6-week-old MPI^118Q/118Q (right) and WT (left) mice. The cerebellar sections were immunostained with Iba1 antibodies and counter-stained with hematoxylin (scale bars indicate 1 mm.)