Figure 7.

Blue Native gel electrophoresis and western blotting with brain mitochondria lysates. Lysates were prepared with brain synaptic and non-synaptic mitochondria from 10-month-old WT and YAC128 mice as described in Materials and Methods. (A) A representative Blue Native gel. (B) Western blotting with anti-Ndufa9 antibody. Molecular weight of Ndufa9 is 39 kDa. However, after Blue Native–PAGE Ndufa9 is detected in the Complex I with molecular weight above 720 kDa (38). (C) The results of densitometry performed with NIH ImageJ 1.48v software. Data are mean ± SEM, N = 6.