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. 2015 Jun 12;24(17):5024–5039. doi: 10.1093/hmg/ddv223

Figure 8.

Figure 8.

Identification of an LHX target cis-regulatory element in Foxp1 locus. (A) LHX6 ChIP-seq result at the Foxp1 locus visualized by IGB. The dotted box (sil) corresponds to Foxp1_sil used in B–F. The GERP scores from UCSC Genome Browser are shown at the bottom. (B and C) ChIP-qPCR from E11.5 maxillary arch cells for Foxp1_sil and an NC region (NC) located 23 kb away. The error bars are standard deviations from triplicates. P-values were calculated between the fold enrichments of NC and Foxp1_sil. *P < 0.05, **P < 0.01. (D) Multiz Alignments of the Foxp1_sil region from UCSC Genome Browser (50). (E and F) Luciferase reporter assay in PMACs from E11.5 embryos. The relative luciferase activity from Foxp1_sil reporter was significantly reduced when co-transfected with an LHX expression vector in comparison with when co-transfected with empty pCIG. The error bars are standard deviations from triplicates. *P < 0.05, **P < 0.01.