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. 2015 Mar 9;77(7):823–828. doi: 10.1292/jvms.14-0655

Table 2. The effects of vaccination on prevention against V. vulnificus infection in mice.

Immunized dose
with (cfu/mouse)		 Dead /Challenged
(Numbers of mice)
Mock (PBSG buffer) 5/5
2.5 × 103 1/5
2.5 × 104 0/5
2.5 × 105 0/5

A signature-tagged mini-Tn5Km2-inserted mutant V. vulnificus, S10T79, was intraperitoneally injected into mice as a vaccine. Vaccinations were given twice with a two-week interval. A week after the secondary vaccination, the mice were challenged with the original strain, V. vulnificus OPU1. The status (dead or alive) of mice was judged after 36 hr post the challenge injection.