In animals that showed greater food intake compared to controls, DHβE injections at 2 mg/kg increased cFos expression in laterally located HO neurons (A1-2) (Saline and DHβE, n = 6) and decreased cFos expression in total MCH neurons (A3) (Saline, n = 4; DHβE, n = 5). There was no difference between 2 mg/kg DHβE and Saline groups in TH A13 (Saline and DHβE, n = 6) and A12 cFos expression (Saline and DHβE, n = 4) (B1-2). Abbreviations: HO, hypocretin/orexin; MCH, melanin concentrating hormone; TH, tyrosine hydroxylase; f, fornix; DMH, dorsomedial hypothalamus; LH, lateral hypothalamus; PeF, perifornical region; PeFLH, perifornical region of the hypothalamus; Arc, arcuate nucleus. Scale bar: A, 300 μm; B, 100 μm.