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. 2015 Aug 6;10(8):e0135087. doi: 10.1371/journal.pone.0135087

Fig 2. Splenectomy does not alleviate the kidney phenotype in NTS.

Fig 2

Subsequently to either sham-operation (Sham, white bars, n = 5) or splenectomy (SplX, squared bars, n = 5), NTS was induced and albuminuria (A) on respective time points and PAS-Scores (B) on day 14 were evaluated. Representative PAS-stained kidney sections of a sham-operated (C) and a splenectomised (D) mouse are shown. Kidney infiltrating CD4+ and CD8+ T cells (E) as well as Ly6G+ neutrophil granulocytes (E) and CD68+ macrophages (F) were evaluated by performing immunohistochemical stainings from kidneys of sham-operated and splenectomized mice. Representative immunohistochemical stainings for CD4+, CD8+ cells and CD68+ macrophages are shown (G). Data are given as means ± SEM and are representative of three independent experiments with at least n = 4 in each group. Magnification x1000 (C, D, G) except for CD68+ (G), where magnification is x600.