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. 2015 Aug 10;3(7):e448. doi: 10.1097/GOX.0000000000000340

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Copyright © 2015 The Authors. Published by Wolters Kluwer Health, Inc. on behalf of The American Society of Plastic Surgeons. All rights reserved.

This is an open-access article distributed under the terms of the Creative Commons Attribution-Non Commercial-No Derivatives License 4.0 (CCBY-NC-ND), where it is permissible to download and share the work provided it is properly cited. The work cannot be changed in any way or used commercially.

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Fig. 3. — Presternal and eyelid cutaneous fibroblasts differ in their cell-force profiles: eyelid fibroblasts and presternal cells were cultured in collagen gels to generate 3D tissue-like matrices, which were then starved overnight in serum-free medium. Cellular force generation and tissue matrix rigidity were determined before (starved) and after stimulation with 20% FBS for 15 minutes (stimulated). A, Eyelid cutaneous fibroblasts display greater intrinsic baseline cellular contractile force when compared with matching presternal fibroblasts under resting conditions (**, P < 0.01), but only presternal cells showed a significant increase in contractile force following stimulation (***, P < 0.001). B, Matrices prepared with eyelid cutaneous fibroblasts displayed greater elastic force than those made with presternal cells (*, P < 0.02), with no change after serum stimulation.